Induction and Selection of Sox17-Expressing Endoderm Cells Generated from Murine Embryonic Stem Cells
نویسندگان
چکیده
منابع مشابه
Induction and selection of Sox17-expressing endoderm cells generated from murine embryonic stem cells.
Embryonic stem (ES) cells offer a valuable source for generating insulin-producing cells. However, current differentiation protocols often result in heterogeneous cell populations of various developmental stages. Here we show the activin A-induced differentiation of mouse ES cells carrying a homologous dsRed-IRES-puromycin knock-in within the Sox17 locus into the endoderm lineage. Sox17-express...
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Background: Human embryonic stem cells (hESC), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. In this study we examine the differentiating potential of hESC into hepatocytes by characterization of the expression of endoderm and liver-specific genes. Methods: hESC were cultivated in suspension to form aggregates, the embryoid bodies. ...
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Human embryonic stem cells (hESCs) can provide insights into development of inaccessible human tissues such as embryonic endoderm. Progress in this area has been hindered by a lack of methods for isolating endodermal cells and tracing fates of their differentiated progeny. By using homologous recombination in human ESCs, we inserted an enhanced green fluorescent protein (eGFP) transgene into th...
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ژورنال
عنوان ژورنال: Cells Tissues Organs
سال: 2011
ISSN: 1422-6405,1422-6421
DOI: 10.1159/000329864